: Sex hormone binding globulin (SHBG) and lipoprotein lipase (LPL) play an important role in erectile dysfunction (ED) in overweight men. This study focuses on the study of the rs727428; rs5934505; rs10822184 single nucleotide polymorphisms that determine the predisposition to erectile dysfunction in overweight men, as well as the relation of these polymorphisms with the level of sex hormones in the Kazakh population. A total of 200 men of Kazakh nationality, after consent, have been selected for the study, of which 70 patients with ED IIEF-5 (6-25) and overweight (BMI ≥ 25) and 130 healthy men IIEF-5 (26-30) normal weight (BMI <25). Single nucleotide polymorphisms rs727428 [C / T]; rs5934505 [C / T]; rs10822184 [T / C], were determined by the TaqMan method. Also, levels of SHBG, total testosterone, and serum albumin were measured. The rs 5934505 SNP is associated with ED affected by obesity (p = 0.004) in comparison with the control group. No association of rs5934505 polymorphism with lipid metabolism and sex hormone levels has been detected. The rs727428 polymorphism affects the level of total testosterone (p = 0.001) and albumin (p = 0.03) while the rs10822184 affects the level of LDL (p = 0.01) and triglycerides (p = 0.04). Thus, rs5934505SNP is associated with erectile dysfunction accompanied by overweight in people of the Kazakh population. This study also reveals that rs727428 affects the level of total testosterone in the Kazakh population.