International Medical Journal

Journal ID : IMJ-31-12-2020-711

Total View : 365

Title : Genetic Polymorphism and Diversity Associated with Sickle Cell Disease Correlating with Complications

Abstract : Genetic modifications in sickle cell disease (SCD) result in clinical diversity. We analyzed the hereditary polymorphism in 45 patients diagnosed with sickle cell anemia (SCA). Patients with SCA presenting at a tertiary care hospital of Eastern India, who were diagnosed based on clinical and hematological examinations were included in this study. The genetic polymorphism was determined using DNA isolation from the patients’ blood samples followed by DNA quantification, amplification through polymerase chain reaction (PCR), sequencing using BLAST X tool and phylogenetic analysis based on MEGA 6 software to estimate the evolutionary relationships of the nucleotide. A total of 60 samples were collected from 45 patients. Of these 60 samples, the DNA isolation and purification resulted in 45 successful PCR amplifications using a gene specific to sickle cell hemoglobinopathy. An unrooted neighbor joining the phylogenetic tree was built from the nucleotide sequence alignment, which showed that some of the sequences were closely associated and some were diverse. The sequence and phylogenetic information associated with goblin chain can be utilized in further research in SCA and other hemoglobinopathies..
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International Medical Journal

Journal ID : IMJ-29-12-2020-709

Total View : 414

Title : Development of a robust blood smear preparation procedure for external quality assessment

Abstract : The external quality assessment (EQA) scheme is particularly important for laboratory performance evaluation. Peripheral blood smears are necessary to identify morphological features, and the procedure for preparing such smears must be robust to meet the ISO 15189 standard. Although blood smear preparation is a routine activity in medical laboratories, an appropriate procedure for preparing a series of blood smears with high homogeneity and durable stability for EQA purposes has not yet been published elsewhere. For this reason, a robust procedure was developed and validated in this study. Various factors affecting blood smear preparation, such as the amount of time collected blood samples are stored before fixation, suitable reagents, and specification parameters for each step, including fixation, staining, and timing of the staining steps, were studied. Each experiment was evaluated based on homogeneity and stability characteristics. Whole blood mixed with EDTA anticoagulant was used to make the blood smears. Samples were fixed with pure anhydrous alcohol and stained in Coplin jars using the Wright-Giemsa method. The homogeneity and stability of two lots of smears suitable for EQA purposes was confirmed based on intact morphology of the smears for more than 8 months at room temperature..
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International Medical Journal

Journal ID : IMJ-29-12-2020-708

Total View : 358

Title : The Expression of interleukin-15 in muscle-effect of exercise on High-Fed-Diet mice

Abstract : This study investigates the effects of exercise on expression of interleukin-15 (IL-15) in muscle of high- fat-diet mice. Mice were divided into 5 groups of 4 in each. Apart from normal control, HFD and standard, mice also received exercise once per week, three times per week, and five times per week for 2 weeks. The expression of IL-15 was evaluated using PCR. The exercise treatment was decreasing the body weight compare with HFD group only. Furthermore, the evaluation of PCR showed that the expression of IL-15 was decreased in muscle of HFD group. However, exercise elevated the expression of IL-15. This study indicated that exercise-induced an increase of IL-15 expression following endurance exercise might be a potent mediator in obese disease..
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International Medical Journal

Journal ID : IMJ-27-12-2020-707

Total View : 392

Title : Diabetes as the risk of Cancer: A Literature Review

Abstract : Cancer is a non-communicable disease with high mortality and morbidity rates. Data recorded by GLOBOCAN in 2018 shows the number of cancer patients is reached 18 million people with a death rate of 9 million people and predicted to increase every year. Diabetes is known to affect morbidity and mortality in cancer patients through increasing cancer cell proliferation and the risk of metastasis. This literature study not only explains the epidemiology, but also the mechanisms underlying the association of diabetes to cancer in general. Literature search was done using four search engines from PubMed, ProQuest, Science Direct and Google Scholar in the last 5 years, but relevant older articles were included. Cancer occurs due to the carcinogenesis process. This process is complex, where normal cells do not immediately mutate and become cancer cells but through several stages. The possible mechanisms underlying carcinogenesis in diabetic patients are hyperglycemia, hyperinsulinemia, chronic inflammation, genetic variation, obesity, and hyperlactatemia. Examination of sugar levels in cancer patients, as well as cancer screening in patients with diabetes need to be done to detect early events and reduce morbidity and mortality..
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International Medical Journal

Journal ID : IMJ-27-12-2020-706

Total View : 406

Title : The Success of E-learning in the Time of Covid-19 Pandemic From the Viewpoint of Jordanian Teachers

Abstract : The COVID-19 has resulted in schools shut all across the planet. Globally, over 2.1 million Jordanian kids are out of the schoolroom. As a result, education has modified dramatically, with the distinctive rise of e-learning, whereby teaching is undertaken remotely and on digital platforms. analysis suggests that on-line learning has been shown to extend retention of data, and take less time, that means the changes coronavirus have caused could be here to remain, there are, however, challenges to beat. Some students while not reliable net access and/or technology struggle to participate in digital learning this gap is seen across Jordan and between financial gain brackets inside it. during this analysis we have a tendency to study the result of e-learning on Jordanian academics, by questioners disclosed that academics aren't pleased with e-learning, due to many reasons, and disadvantages..
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International Medical Journal

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Submission Deadline ( Vol 30 , Issue 06 )
20 Jun 2023

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Publish On ( Vol 30 , Issue 05 )
31 May 2023