Abstract : This study address the current knowledge and attitude of Yemeni dental practitioners accomplished during the root canal procedure and subsequent therapy in the treatment of acute apical periodontitis of the tooth. This cross-sectional study was conducted on 156 randomly selected general practitioners who worked in dental clinics in Sana’a city, Yemen. The data produced by the study was analyzed using the Chi-squared test with a significance level set at p < 0.05, which yielded a reasonable determination as to whether or not there was a significant relationship between the study’s core variables. Antibiotics were prescribed by 93.58% and anesthesia administration by 78.2% of the general practitioner participants in the study. The protocol with the highest rate in the study was multiple irrigation, at 69.23% of participants; while rubber dam usage was the lowest: only 0.64% of the participants employed this technology compared to other isolation methods. Overall, 57.05% of practitioners reported manipulating both hand and rotary means in cleaning and shaping the root canal system; 48.71% used a calcium hydroxide intracanal medicament; 44.23% a mixture of both calcium hydroxide and chlorhexidine; and 39.1% sodium hypochlorite, hydrogen peroxide, and chlorhexidine, significance differences were found between the participants in all clinical procedures (p < 0.05) except in isolation method and selection of intracanal irrigants (p > 0.05). According to the findings of this study, neither the type of work nor the experience period influenced the clinical attitude and/or approach of dentists in endodontic therapy. The mechanical method selection for root canal system preparation meanwhile, showed variation.