Abstract : Hepatitis has become a foremost health concerns worldwide. Majority of the infected population remain unaware of their disease status due to non-screening practices. The aim of present study was to evaluate the prevalence of undetected cases and practices regarding Hepatitis B & Hepatitis C among patients attending community pharmacies of southern Punjab, Pakistan. A cross sectional study was conducted for the duration of three months from January to March, 2017 using a structured, validated questionnaire. Rapid diagnostic kits were utilized for screening of patients from selected community pharmacies of two big cities of South Punjab, Pakistan. Descriptive and inferential statistics were applied for data analysis by using SPSS version 20.0. P value <0.05 was considered to be statistically significant. Among total 794 respondents, 565 (71.2%) were males and 229 (28.8%) were females. Prevalence of undetected HBV cases was 2.01% (n = 16) and for HCV was 5.41% (n = 43). Results showed poor practices of respondents with mean practice score of 2.54/6 (±1.581). Study emphasized the need of educational programs along with motivational campaigns to persuade people for regular screening of HBV & HCV that will prove beneficial in early diagnosis and management of the disease.