Abstract : Neonatal sepsis is one of the most common infections occurring in the neonatal intensive care unit, often a challenging emergency situation, which if not managed promptly can result in neonatal death. The aim of the study is to determine, causative organisms found on newborn skin by collecting skin swabs once a week so as to understand the changing pattern of microbial flora and their resistance to commonly used antibiotics, especially when the baby shows a high WBC count or a deteriorating chest x-ray. A prospective study was conducted in the Neonatal intensive care unit of a Medical college Hospital and research center, after the approval of the Institutional Ethics Committee (IEC), the study was conducted on 100 newborns. Skin and groin swab samples were collected from the newborns with basic aseptic precautions, after informing them and obtaining consent of the mother or guardian. Around two swab samples were taken from the groin and armpit, one on day-1 and the other on day-4 and sent for culture sensitivity. The reports of the samples were collected and compared to observe the changing trend of the bacteria and the resistance shown to commonly used drugs. Coagulase negative staphylococcus and E.coli were the most common causative organisms in our area, based on the analysis of both days taken, apart from that Methicillin Resistant Staphyloccocus and Klebsiella were the second common bacteria prevalent in the area.