Abstract : The induction of the type II diabetes model in Sprague Dawley rat is commonly performed for further laboratory experimental purposes. The main characteristic of type II diabetes mellitus is insulin resistance, which highly associated with high-fat and high-sugar diets. Therefore, to develop this condition, the current study proposed an alternative procedure of induction, wherein includes a combination of a high-fat diet with multiple injections of streptozotocin and immediately, 5% glucose drinks were administered after the injection to enhance the hyperglycemic and insulin resistance state in the rat. This protocol successfully developed the hyperglycemic state. Poor glucose clearance indicated insulin resistance in the rat.  In conclusion, this protocol provides an alternative procedure in inducing insulin resistance and a hyperglycemic state of type II diabetes mellitus in Sprague Dawley rat