Abstract : <p>To determine the antibacterial activity of a mixture of Sphatika, Navasadara, Suryakshara against common urinary pathogens. Evaluation of Sphatika, Navasadara, Suryakshara against common urinary pathogens and compare the antimicrobial activity of the Ayurvedic medicines with the standard urinary antibiotics. The material includes Mullor Hinton Agar, Sterile cotton swab, Sterile plane discs, Forceps, Sterile test tubes, Sterile cotton, Conical flasks, distilled water, Autoclave, Hot air oven, Incubator and the test organisms include Escherichia coli, Klebsiella pneumoniae, Proteus species, Pseudomonas aeruginosa, Serratia species, Staphylococcus aureus. Kirby Bauer disc diffusion method was employed to determine the antibacterial effect of the drug. Escherichia-coli, Klebsiella pneumonia and Serratia species showed sensitivity at 2000mg, 3000mg, and 3500mg but were resistant to lower concentrations. At the same time, staphylococcus aures and Proteus vulgaris exhibited resistance to the formulation of all the concentrations with a slight irrelevant zone with higher two concentrations. The formulation is a choice for treating urinary tract infection, considering its nil side effect, and further studies must be in place for advancement.</p>