Abstract : A.actinomycetemcomitans are pathogenic bacteria that cause damage to periodontal tissue so that it is clinically seen as a periodontal disease (periodontitis). There are no studies that report bacterial load after the use of purple miana leaves extract (PMLE) in Periodontitis. This research objective was to determine the effect of purple miana leaves extract on bacterial load in wistar rats that have been induced by A.actinomycetemcomitans. Rats were divided into three groups, purple miana leaves extract (PMLE) 510 mg/ kgbw, negative control (aquadest), antibiotic levofloxacin 500 mg/kgbw as positive control. Samples of A.actinomycetemcomitans bacteria were taken seven days after induction of 3x108 cfu/ml A.actinomycetemcomitans in gingival sulcus of the anterior mandibular teeth of the rat/after periodontitis (D8) and seven days after intervention (D15). Bacterial load is measured by calculating colony forming units. The results obtained are processed using SPSS. Bacterial load on D8 to D15 had a different pattern between the PMLE group, the aquades group (negative control) and the levofloxacin group (positive control). In negative controls, an increase in bacterial load but not significantly different (p>0.05). In PMLE and positive control, there was a decrease in bacterial load and significantly different (p<0.05). Purple miana leaves extract (PMLE) 510 mg/kgbw and levofloxacin 500 mg/kgbw successfully suppressed the growth of A.actinomycetemcomitans (bacterial load) colonies for 7 days of use.This study represents that PMLE can be an alternative drug in patients with A.actinomycetemcomitans infection, especially in the case of periodontitis