Vol - 25, Issue - 3
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[This article belongs to Volume - 25, Issue - 3]
International Medical Journal
Journal ID : IMJ-05-03-2020-324
Total View : 149

Abstract : In this study, fifty isolates of S. marcescens were isolated from Blood, wound, Sputum and urinary tract infections, the results of screening the highest and best bacterial isolates for producing prodigiosin pigment was done and the isolate (SKT14) gave the highest pigment production. Prodigiosin pigment was extracted using ethyl acetate and acetone , Purification was done by using column chromatography , Minimum inhibitory concentration method was used to evaluate the inhibitory effect of purified prodigiosin pigment and the result showed that MIC was 1.56 μg/ml against Enterobacter sp , 25 μg/ml against S. marcescens and 50 μg/ml against S.aureus , as well as by using turbidity method the highest inhibition rate at concentration 50 μg/ml was seen against S. aureus and Enterobacter sp. , moderate against E.coli , while the lowest inhibition rate was against K.pneumoniae and S.marcescens , while highest inhibition rate at concentration 1.56 μg/ml was seen against S. aureus and E.coli , moderate against Enterobacter sp. and S.marcescens , while the lowest inhibition rate was against K.pneumoniae

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