Abstract : University students face numerous types of academic stress like tests, theoretical and practical coursework, research activities and various aspects of professional practice. Since stress levels highly affect ANS function, the aim of the present study was to assess the autonomic nervous system function among GMU students by using autonomic reflex tests such as cold pressor test, hand grip test and heart rate variability. From the results, there was a significant association between gender and HRV as well as gender and diastolic blood pressure for isometric hand grip test. For HRV test, a positive correlation between gender and HRV was found where females had a median average score of 74 while males had an average of 80. Furthermore, the total median average of HRV among the participants was 78 which suggests overall that the participants were healthy and in good condition. As for physical activity, smoking and age all had no correlation with HRV since there was no significant association with any. The HR mean value was (91.59±17.605) beats/min and following CPT it increased to (94.24±16.933). The mean value for SBP at resting was (123.53±16.200) and following CPT it increased to (125.33±18.001). The resting DBP mean was (79.32) and following CPT it increased to (82.59). In Isometric handgrip test, there was no significant association between SBP changes and gender/physical activity/smoking. Only 17 students showed hyperactivity based on SBP changes, 9 were males and 8 females. We found a significant association between DBP changes in IHGT and gender only. The results in the study showed that the majority of the students are healthy. ANS testing must be done on students for early detection of dysautonomia.