Abstract : COVID-19 is a major Public Health challenge that has affected the world’s economy. Assessment of the knowledge, attitude and practices of Healthcare workers (HCWs) towards COVID-19 can improve or sustain successes being recorded by relevant agencies in the fight against COVID-19. This study sought to determine the levels of knowledge, attitude of HCWs towards COVID-19 among Healthcare workers at the Alex Ekwueme Federal University Teaching Hospital, Abakaliki. A cross-sectional survey was done using semi- structured questionnaires which were pre-tested for clarity and a simple sampling technique. Data collected were analyzed using SPSS version 22. Categorical variables were presented as frequencies and percentages while continuous variables were presented as mean and standard deviation. Out of the 368 respondents, 205 (55.7%) were doctors while 150 (40.8%) were nurses. Others were clerical workers, administrative officers and maintenance workers. The sex distribution showed 181 (49.2%) were males while 187 (50.8%) were females. The 25-34 years’ age group was the modal age group. Knowledge of COVID-19 was generally high amongst respondents recording a correct response to questions about knowledge of COVID- 19 in more than 90% of the case. However, the attitude and practice of COVID-19 infection, prevention and control protocols were generally poor especially for this group of the population who are Health care professionals. Out of the respondents, 41.8% would not stay at home if they had minor COVID-19 like symptoms, only 39.4% would take responsibility to ensure people around them follow good respiratory hygiene. Respondents who would keep social distancing while talking with co-workers, ensure safe disposal of personal protective equipment (PPEs) and safe waste management of PPEs were 56%, 39.9% and 53.8% respectively. This study showed that knowledge of COVID-19 disease prevention protocols is high but attitude/practice of these laid out protocols still desires a lot of effort from HCWs. There is need for training/retraining of HCWs on COVID-19 infection, prevention control strategies to ensure the success being recorded against the disease is sustained while preventing the possibility of a second wave of infection and to be armed with relevant skills to reduce infection amongst co-workers and the public if it does occur