Abstract : The rapid spread of COVID-19 has caused challenges worldwide and affected the mental status of the population including nurses. Nurses play a vital role in dealing with COVID-19 victims. This study was aimed to determine nurses’ awareness and perception towards infection control measures against the COVID- 19 pandemic in Saudi Arabia. The hospital-based cross-sectional study design and the data were collected from all regions of Saudi Arabia from May to June 2020 through a self-administered questionnaire. Descriptive statistics included frequency distributions with percentages. In addition, the study evaluates the differences in the level of knowledge; practice and attitude in association of socio-demographic variables between the nurses were applied by Chi-square test. A total of 534 nurses were participated in this study resulting in 71% response rate. Of the participants 201 (37.6%) were Saudi nationals and 333 (62.4%) were non-saudi nationals The findings showed that nurses and nurse interns in Saudi Arabia have a high level (85%) of knowledge and a positive attitude towards Covid19 outbreak. It also reveals that participants are highly satisfied (84 %) toward hospital managements and patients’ reception (91 %) to combat COVID-19 in Saudi Arabia. On the other hand, there is statistically significant difference (P-value < 0.05) present between Saudi, non-saudi nurses and area of work location in association of level of knowledge, practice and attitude toward COVID-19. There is a clear need for training programs to improve the understanding of the risks and prevention strategies among nursing professionals for infection control during COVID-19 pandemic in Saudi Arabia