Abstract : Contamination of hospital water supply with potentially pathogenic organism can occur from various environmental sources leading to serious implications when used for various activities of parient care. The intensive care units are most frequently involved setting but high risk immunocompromised patients can be affected irrespective of ward. Hence the present study was conducted to assess the extent of bacterial contamination among water sources used by healthcare professionals. 50 tap water samples from various areas of Medical College and Hospital were collected. 100 ml sample was passed aseptically through 47 mm diameter and 0.45 μm-pore-size cellulose nitrate sterile membrane filter grid held in a filtration unit. This transferred to MacConkey and blood agar plate and incubated at 37o C. Total number of colonies were counted as colony forming units. Bacteriological analysis of water sample from operation theatres found satisfactory. Paediatric ICU 1 & 2 showed 12 and 8 colonies/100 ml respectively and Klebsiella pneumonia was isolated. Similarly, Pseudomonas spp from obstetrics and gynaecology ward, Klebsiella pneumoniae from male orthopaedic ward while Acinetobacter hemolyticus from neurosurgery ward were isolated with significant colony count. Regular disinfection and bacteriological assessment of all water sources are important in controlling healthcare associated infections.