Abstract : The end-stage renal disease (ESRD) in Indonesia continues to increase. This causes an increase in the need for kidney transplants. Prior to 1994, open donor nephrectomy (ODN) was considered as a standard procedure for kidney donors. Open donor nephrectomy is a safe operation but this technique is associated with significant morbidity in terms of disability, cosmetics, leg injuries, incisional hernias, neuralgia, and lower back recovery. Higher procedure costs are also a major problem. Mini-incision donor nephrectomy (MODN) has now been developed as an option for donor nephrectomy. MODN has advantages including less postoperative pain, early recovery and fewer complications and later incisional hernias. The purpose of this study was to examine the number of arteries & estimated Glomerular Filtration Rate (eGFR) with the MODN surgery time. This is an observational, cross sectional study. Data collected from the medical records of patients who were transferred kidney transplants from January 2014 to December 2018 at the Kariadi General Hospital Semarang, Indonesia. There are 20 patients, 15 males and 5 females. Total donor relationships with related recipients were 15 patients, 5 were unrelated. The average ischemic time was 38.65 ± 1.81 minutes (range 36-42). Total patients do not need a blood transfusion after a kidney transplant. The average operating time is 58 ± 95 minutes (range 55-63). The average number of Arteries involved was 2.55 ± 0.69 (range 1-4), the average value of eGFR donors after surgery was 87.84 ± 1.36 (range 84.51 - 89.44). Data were analyzed by the Spearman test in SPSS version 23. This study showed that the operating time was significantly correlated with the number of arteries & eGFR (p <0.001). In conclusion, the operating time has a significant amount with the number of arteries & eGFR. The faster the time required for surgery, the higher the eGFR after surgery should have better results, and the fewer arteries involved during surgery will heal faster and will not cause complications