Abstract : Enzymes are biological catalysts (also known as biocatalysts) that in living organisms speed up biochemical reactions and can be extracted from cells and cells. A large range of commercially relevant processes were then used to catalyze. Current work is an attempt to develop a new spectroscopic method for quantifying thymol in large quantities and in its pharmaceutical component. The projected methodology is depended on the oxidative coupling of thymol with 2-aminophenol in the attendance of hydrogen peroxide additionally enzyme peroxidase to create a colored compound owing a maximum absorption at 436 nm. The reaction conditions are optimized to obtain the maximum color intensity. It was found that the absorbance increased linearly with increase of the concentration of thymol. Systems comply with Beer's law in the kind (2- 44 μg / ml). Correlation coefficient values were creating to be 0.9994 Sandell sensitivity was calculated as 0.057 μg /𝑐𝑚2. The results of these methods' analysis and recovery studies were statistically validated. The method is applied to pharmaceutical preparations. The percentage of relative standard deviations is 0.144. The accuracy was checked by carrying out recovery experiments and found to be 102.4,100.05,100.2. Excipients typically present in pharmaceutical formulations did not cause any interference. The methods chosen are sensitive, clear, and repeatable, and can be used to evaluate thymol in various pharmaceutical dosage forms on a regular basis.