Abstract : An estimation of five parameters viz, (FBS, PPBS, serum urea, creatinine, & uric acid) related to diabetic patients was carried out, and the concerned parameters are neither very sensitive nor very specific markers of renal function. As per the study it had been found that main biochemical markers slightly affect serum uric acid, serum urea & serum creatinine, which may represent risk factors in type II Diabetic patients. As per the results it had been observed that male Diabetic patients have higher levels of Serum Urea, Serum Creatinine & Serum Uric Acid than female patients. So the tendency of occurrence of renal function test value at the higher reference limits in case of Type II Diabetes mellitus reflects the initiation of nephropathy changes. As per the results it could be mentioned that serum creatinine, serum urea and serum uric acid may be essential for early detection of diabetic and nephropathy. Hence these tests should be a part of routine checkup for diabetic patients and healthy volunteers