Abstract : Hepatitis C virus infects liver and degenerate its structure. The progression of disease to chronicity is leaded by the production of acute phase proteins mainly CRP. C reactive protein is synthesized in liver and stimulated by cytokine Il-6, in response to tissue damage, inflammation and infection. The objective of this study was to find the association between CRP and HCV in patients with both positive and negative HCV RNA by PCR. A total of 98 HCV positive patients from Best-way Medical Center, Lahore, and Project of WHO at Allama Iqbal Medical College, Lahore and CAMB were included in the study in which HCV antibodies were detected to be positive. These all patients were tested for the detection of viral RNA by PCR. CRP assay was performed to all specimens of patients. According to study, 50(51%) out of 98 were positive for HCV RNA and 48(49 %) out of 98 were negative for HCV RNA. 6 (12 %) out of 50 were positive for CRP in HCV RNA positive cases while 12 (25 %) out of 48 were positive for CRP in HCV RNA negative cases. The finding of present study shows that there is no significant relationship of CRP and HCV infection and more, that CRP assay is not useful as a diagnostic marker of hepatitis C virus