Abstract : To evaluate cytotoxic and antiviral potential of Moxidectin and Amantadine against influenza virus H9. The cytotoxicity of moxidectin and amantadine was evaluated on primary fibroblast cell line through MTT assay. Antiviral activity in vitro and in vivo was tested on 9-days old chick embryo. Cytotoxicity and antiviral activity of Moxidectin is high at first two conc. 200µg/ml and 100µg/ml while safe at lower conc. 50, 25, 12.5 and 6.25µg/ml. Antiviral activity of Amantadine is strong at all conc. 200µg/ml, 100µg/ml, 50µg/ml, 25µg/ml, 12.5µg/ml and 6.25µg/ml. All the concentrations of Amantadine were non-cytotoxic with higher safety profile. It was concluded that concentrations of moxidectin 200 and 100µg/ml were cytotoxic and lower concentrations were safe. All the concentrations of Amantadine were non-cytotoxic. Moxidectin at 200 and 100µg/ml demonstrated strong antiviral potential and antiviral potential decreased as concentrations decreased. Amantadine showed strong antiviral activity against influenza virus H9 at all concentrations.