Abstract : This study is performed to assess the correlation between some serum cytokine levels with vitamin D status as well as the bone mineral density in blood samples obtained from 100 Iraqi β-thalassemic major matching in age and gender with 100 healthy control subjects whom collected from Al- Alkarama Teaching hospital during December 2018 to August 2019. Measurement serum levels of Transforming growth factor -β, Interleukin -35,Tumor necrotic factor-α, and vitamin D were done using ELISA kits, as well biochemical measurements of serum alkaline phosphatase, inorganic phosphorus, in addition to the serum calcium have been performed through conventional colorimetric methods.Furthermore,the results indicated significant alteration in all serum cytokines studied levels as well biochemical parameters in the β-thalassemic major patients when compared to the healthy control subjects. Our information suggests the presence of severe deficiency of vitamin D, in addition to the imbalanced immune conditions including immunosuppressant and inflammation in the thalassemia β-major patients, that might be changed to extra efficient immune response through the intake antioxidants