Abstract : Unfortunately, the risk of polio virus importation and spread remains high in Sudan, due to previous history of WPV importation to HOA from Nigeria and persistently low RI coverage. [67]. The knowledge of parents about immunization is a public health intervention that has greatly reduced mortality and morbidity globally [16]. o assesses the impact of awareness and health education on determinants of parent’s decision to vaccinated their children against poliovirus, the parent’s knowledge and attitude were also assessed pre-and post-intervention for those who received the health education (information sheet) about poliovirus disease and vaccine. A quasi-experimental pre- and post-facility-based study, the present study was conducted in three Sudanese cities (Khartoum City, Omdurman City, and Khartoum North city). total coverage of all parent’s during the period of data collection the number of participants in this study was 426. The data were collected by using the quantitative method through questionnaire to measure the score of knowledge regarding polio disease and vaccine, attitude of parents and vaccination status of their children based on construct of the theoretical model. The collected data was coded, organized, tabulated and analyzed. descriptive data was presented in table, pie and column. Chi-square was used for correlation between deferent variables, t-test was used for comparison between groups and pre- and post-intervention. For all tests, the significance level was set at p ≤ 0.05 In comparison between total parent’s knowledge among pre-and post, it was noticed that there is statistically significant difference between parent’s knowledge after post -test. P < 0.05 parent’s general attitude among pre-and post, it was also noticed there is statistical significant difference between parent’s general attitude to vaccine after post- test. P < 0.05. The internal reliability of the five constructs pertaining to the theoretical model was strong. Cronbach’s alpha was > 0.70. Chi-Square shows there is no statistically significant relationship between the five constructs pertaining to the theoretical model pre- and post-intervention P value = 0.859> a = 0.05. In a question on parental sources of information about vaccination, most parents cited their doctor (62.2%), for parents of vaccinated children compared with (52.4%) for parents of unvaccinated children. Chi-Square shows there was slightly increase in score of knowledge about poliovirus disease. (post- intervention) P < 0. 005 A small percentage of the parents had a knowledge deficiency regarding poliovirus disease However parents had not vaccinated their children against poliovirus in all cities ، and that because they believed their children was receiving enough vaccines, acceptability of a vaccine and successful implementation of immunization programs are critically dependent on parental knowledge, attitudes and beliefs regarding the disease and the vaccine. By raising awareness of parents، Parents decided to vaccinate their children. Activation the health care provider’s roles in awareness and health education parent’s awareness toward vaccination should be implemented as routine in vaccination days. Use of civil society and community leader’s efforts to increase the parent’s awareness about the importance of vaccination in reducing morbidity and mortality.