Abstract : This study aimed to assess the ions released from two brands of orthodontic bondable molar tubes after immersion in different mouthwashes. Eighty orthodontic molar tubes; 40 from Orthotechnology® Company and 40 from IOS® Company were immersed in three types of mouthwash (two herbal and one chemical) in addition to distilled water; ten of each type were immersed separately in each media under 37°C for 45 days. Assessment of the ions released was performed using Atomic Absorption Spectrophotometer and comparisons between the brands and among various mouthwashes were determined by independent sample test and one-way analysis of variance respectively. The results showed statistically highly significant differences in ions released among different types of mouthwashes for both brands; moreover, the tubes from IOS® Company released significantly more ions than that from the Orthotechnology® Company. The number of ions released in herbal mouthwashes was higher than in chemical one, so prolonged use of these mouthwashes is not recommended