Abstract : Biofilm producers causing apical periodontitis in orofacial cleft are the leading dental problem. Present study aimed to focusing Periapical tissue healing in endodontic therapy using Garcinia imbertiBourd. Rootcanal biofilm producers Enterococcus faecalis and Staphylococcus sciuri were screened. Antibiofilm and Antivirulent study were conducted using ethyl acetate fraction of G imbertibourd leaves extract and its purified compound by in vitro method. Root canal model experiments with macrophage interaction in biofilm groups were conducted to study the healing effect of engineered bioactive silver nanoparticles (AgNp). According to MBIC, (MBIC50; 0.25 mg/ml) 60.6% and 61.25% of biofilm was inhibited at 12 and 24 h interval and Ethyl acetate fraction significantly (P<0.05) reduced biofilm growth (90.46%). EPS of E.faecalis biofilm (367.66 µg/ml) reduced after 24hrs (109.34 µg/ml), similarly in S. sciuri biofilm, the total EPS (387.67 µg/ml) was reduced in to 129.345 µg/ml after treatment. Plant extract along with antibiotics found to have promising anti biofilm activity. Also this extract showed the presence of alkaloids, phenols and saponins. AgNp treated biofilm group was found to reduce the proinflammatory markers (nitric oxide, TNF-α, and IL-6 IL-1β), as well as increased anti-inflammatory marker TGF-β1 Engineered AgNp concomitantly inhibited biofilm and rehabilitated the inflammatory response of macrophages that would promote healing. Ethyl acetate fraction of Garcinia imberti bourd found to have significant antibiofilm as well as antivirulence activity against E. faecalis and S. sciuri similarly, rootcanal modeled study concluded that synthesized AgNp helps to modify the microenvironment to be more conducive to periapical tissue healing.