Abstract : Lassa fever (LF), an acute and sometimes fatal viral hemorrhagic disease remains a public health challenge in endemic areas in West Africa including Nigeria. Foodstuff and objects contaminated with multimammate rat, (Mastomys natalensis), droppings or urine is the most common route of disease transmission. Secondary, person-to-person transmission can also occur through exposure to blood or bodily secretion of infected persons (dead or alive). The study was carried out amongst the undergraduate students of university of Nigeria, Enugu Campus to assess the level of knowledge, attitude and practice of Lassa Fever prevention in the student community. A descriptive cross-sectional study was carried out with a sample size of 300 undergraduate students of University of Nigeria, Enugu Campus, (UNEC). A multistage sampling technique was used for sample selection. A simple random sampling method was used to select nine departments from fifteen departments in UNEC and 300 students were then randomly selected from the nine departments. They all met the inclusion criteria. A semi-structured self-administered questionnaire was used for quantitative data collection. Data was analyzed using statistical package for social sciences (SPSS) version 23.0. Data was presented using tables, pie charts and bar charts. The study had a 96% response rate. Results showed that 97% of the respondents have heard of Lassa fever prior to the interview and 62% had knowledge of survival and possible recovery from the disease. The major sources of information amongst our respondents were Radio (53%), television (55.3%) and Blog/Newspaper/Social Media (50.3%). The majority of respondents (70.3%) had a fair attitude towards Lassa fever Virus(LFV) prevention. Although the results showed a fair level of preventive practices amongst University students, the majority of respondents (61%) reportedly declined to participate in a Lassa fever vaccine trial showing that the attitude as well as practice towards Lassa Fever prevention is not corresponding with the level of knowledge demonstrated by the students. Most respondents got their information from television/Newspaper/Social Media, thus supporting the need for continuous campaigns and news items in the public media to sustain the dissemination of information on Lassa fever in order to correct the high knowledge fair attitude mismatch which is necessary for Lassa fever prevention.