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Vol - 30, Issue - 08
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[This article belongs to Volume - 30, Issue - 08]
International Medical Journal
Journal ID
:
IMJ-13-08-2023-1632
Total View
:
427
Title
:
MOLECULAR CHARACTERISATION AND PHYLOGENETIC ANALYSIS OF SHEEPPOXVIRUS RPO30 GENE ISOLATES FROM MOROCCAN SHEEP
Abstract
: Sheeppoxvirus (SPPV) and Goatpoxvirus (GTPV) are classified as causal agents of notifiable viral diseases. They belong to the genus Capripoxvirus (CaPV) with the Lumpy skin disease virus (LSDV). Although SPPV and GTPV have host preferences, certain strains may infect sheep and goats. Capripoxvirus (SPPV, GTPV, and LSDV) are antigenically related but genetically distinct and require molecular analysis for differentiation. In this study, the RPO30 gene from six isolates identified in Capripoxvirus outbreaks in different parts of Morocco between 2018 and 2021 were genetically and phylogenetically analysed to describe their genetic relationship. Sequential analysis revealed that the percentage of RPO30 nucleotide identity ranged from 93% to 99%, with CaPVs isolates from other countries. The constructed phylogenetic tree classified the six CaPVs detected as GTPVs. Additional analyses and alignments must be undertaken to facilitate the diagnosis and control of Sheeppox in Morocco.
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