Vol - 25, Issue - 6
About the Journal
[This article belongs to Volume - 25, Issue - 6]
International Medical Journal
Journal ID : IMJ-14-05-2020-463
Total View : 248

Abstract : Diarrheal disease is considered a significant health problem, principally in developing countries as it is one of the leading causes of morbidity and mortality especially in infants. Studies found that STEC strains are among the most predominant causative agents in acute diarrhea. The current study aimed to investigate the presence of certain virulence factors and antibacterial susceptibility profile of STEC originating from diarrheal patients of Iraq. A total of 161 human of different ages suffering from diarrhea between August 2019 to February 2019 were registered in this study. Standard bacterial cultural and biochemical methods were used to isolate E. coli species while, PCR was applied to distinguish some virulence genes (stx1, stx2) of STEC isolates. A total of 19 (12 %) isolates were identified as STEC bacterium, and 142(88 %) isolates were non-STEC. Nine isolates of the STEC serotypes were O157. Susceptibilities of 19 STEC isolates were determined for 16 antibacterial drugs. STEC O157 strains and STEC Non-O157 exhibited highest resistance to ampicillin (100%), and (63%) resistance to Cephalosporin group and low resistance (5.6 %) to gentamicin and Amikacin. Also results showed that STEC were the major causes of diarrhea that showed resistant to commonly used antibacterial agents

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