Abstract : The aim of the current study was to investigate the T. evansi infection of dromedary camel, using the molecular tools in comparison to conventional methods. Jugular vein blood samples were randomly collected seasonally for three successive seasons from 500 camels in Nyala area (South Darfur State) and examined parasitologically by Giemsa stained blood smears (GSBS) for the presence of the trypanosomes, serologically for detection of anti-trypanosomal antibodies by card agglutination test (CATT) and molecularly for detection of the T. evansi amino- acid sequence through PCR by using T. brucei spp specific primers. Out of 500 samples 55(11%) were positive by smears, and 140(28%) were positives by CATT through anti-trypanosomal antibodies, while 200(40%) were positive by PCR. The obtained results showed that PCR have higher sensitivity and specificity (95%), while CATT and smears gave less sensitivity 70% and 32% respectively. These findings were useful in formulating strategic control programs, because this new molecular approach will make it possible to detect infections in the very early stages where microscopic examination is unclear and to monitor groups of animals after trypanocidal treatment. This will not only be beneficial for diagnosis also useful for epidemiological study and designing rational control program