Abstract : One of the most common dermal diseases and public health concerns is cutaneous leishmaniasis caused by Leishmania spp. The objective of the present study was to identify the Leishmania species causes cutaneous leishmaniasis directly in biological samples using multiplex PCR in patients of displaced camp, taking into consideration both age and site of lesion. During the period from October to the end of December / 2019 , a total of 76 infected patients with single lesion were underwent analysis including 31 female and 45 male aged 20-53 years old, scrap samples from dermal lesions on face or arms were prepared for DNA extraction for multiplex polymerase chain reaction PCR. Results revealed that Leishmania spp. was detected in 63 sample (82.89% ) included the infection rate of Leishmania.tropica 68.2% (43out of 63) and of Leishmania major 31.7% (20 out of 63).On the other hand , male showed higher infection rate 60.3% (38/63) than females 39.6% (25/63). Also, 13 samples didn't get any amplification (17.1%). For the site of lesions, higher percentage of frequency 65% was shown in face while low percentage recorded for arm 22.9%. This study concluded that methods based on DNA analysis, like multiplex polymerase chain reaction (PCR), are reliable for the diagnosis and identification of Leishmania species. As well, determination of the geographic distribution of specific species caused is important in epidemiological studies because it allows and appropriate control measures