Home
About Us
Information & Guidelines
Article Processing Charges
Open Access Policy
Terms and Conditions
Privacy Policy
Information For Authors
Information Editorial Board
Contact Us
FAQ
Paper Submission Guideline
Register
Login
Submit Now
Vol - 25, Issue - 8
Full-Text PDF
About the Journal
Download
[This article belongs to Volume - 25, Issue - 8]
International Medical Journal
Journal ID
:
IMJ-02-08-2020-556
Total View
:
337
Title
:
NLRP3 inflammasome gene expression and activation in leukocytospermia and non- leukocytospermia in fertile Iraqi men
Abstract
: Inflammasome activation cytokine were associated with bad seminal fluid analysis parameters among infertile men with spinal cord injury suggesting pivotal role of NLRP3 pathway in male infertility. Objective: the present study aims to measurement of NLRP3 inflammasome mRNA expression and their activation cytokines (IL-1ß and IL-18) mRNA expression in semen samples in leukocytospermia and none leukocytospermia infertile men. Methods: semen specimen were collected from 264 infertile men; conventional semen analysis was done according to World Health Organization 2010. Total RNA was extracted from whole semen cells pellet then the relative mRNA expression of the NLRP3, IL-1ß and IL-18 in semen specimens was determined by using qRT- PCR. NLRP3 and caspase-3 proteins in both sperm and leukocytes were detected by Immunoperoxidase staining. Results: there was no significant differences in the amount of NLRP3, IL-1B and IL-18 mRNA expression and NLRP3 protein in the sperm cells between leukocytospermia and non-leukocytospermia infertile men (P>0.05). In contrary there was high significant difference in the NLRP3 protein in leukocytes between these two groups (p≤0.001). While, translated protein of caspase-3 in both sperm and leukocytes was no significantly differed between these two groups (P>0.05). Conclusion: Current study suggests that the NLRP3, IL-1ß were expressed in cellular components of semen sample and their expression unrelated to the state of leukocytospermia, while po-IL- 18 is constitutively expressed in leukocytes, epithelial cells and defective sperm cells and released from these cells into seminal fluid after undergoing to apoptosis and necrosis pathways
Download Info
×
Paper Access Key
Access Key
Submit
No Access Key (
Request for Download
)
Name
Email
Country
Send Request
Our Certificates
Guidelines
Information For Authors
Information Editorial Board
FAQ
Further Information
Article Processing Charges
Open Access Policy
Terms and Conditions
Privacy Policy
//