Abstract : Kidney transplantation is the choice to improve the quality of life of patients. Open Donor Nephrectomy (ODN) is defined as a retroperitoneal procedure performed via a long flank incision on the patient in the lateral decubitus position. The disadvantages of this technique are large scars, relatively long immobilization of the donor, and the risk of muscle relaxation of the flank in patients. Mini-incision Donor Nephrectomy (MODN) has been adopted as a less invasive modification of classic ODN and has proven to be as safe as conventional lumbotomy for donor nephrectomy with visible advantages. Recent studies suggest that longer operation time in MODN may increase hospitalization. The aim of this study is to evaluate the long of operative time of MODN, to access its contribution for the length of hospitalization, and also to evaluate another variable characteristic outcome in MODN. This is an observational and cross sectional study. The data was collected from medical record of patient who underwent kidney transplantation from January 2016 to December 2019 at Kariadi General Hospital Semarang, Indonesia. There were 20 patients which consisted of 15 men and 5 women. Total family-related donor were 15 patients and 5 were not related at all. Mean ischemic time was 38.65 ± 1.81 min (range36-42). All patients did not undergo transfusion after having the kidney transplantation. Mean operative time was 58 ± 95 min (range 55-63). Mean length of hospitalization was 3.45 ± 0.51 days (range 3-4). Operation time was compared with the length of hospitalization. Data was analyzed by using Spearman test in SPSS version 23. The study showed that operation time was significantly correlated with hospitalization time (p < 0.001). In conclusion, operation time has a significant correlation with hospitalization time. Mini Open Donor Nephrectomy had several characteristics such as shorter hospital stay, better cosmesis, and cost-effective than Open Donor Nephrectomy. Other variable outcome characteristics such as gender - which must be related between donor and recipients, age - which should be under 60 years, and ischemic time which MODN is shorter than ODN