Abstract : <p>Temporomandibular joint (TMJ) dysfunction occurs due to degenerative changes in bony parts of jaw joints. Patients of TMJ dysfunction present with complaints of limited mouth opening and pain. Platelet Rich Plasma (PRP) provides a new horizon and helps in improving the treatment opportunity for early TMJ dysfunction.<strong> </strong>This study was aimed<strong> </strong>to determine the effect of PRP injection in the management of this condition.<strong> </strong>A quasi-experimental trial was conducted at Department of Dentistry, Sandeman Provincial hospital, Quetta for 6 months. Samples of 68 candidates were included in the study who were diagnosed with this disease. PRP injection was applied in the TMJ. Pain intensity was noted at baseline before and 3 months&rsquo; injection for each candidate using visual analogue scale. Mouth Opening was also measured by measuring the distance between the two incisors at baseline and after 3 months after receiving injection. SPSS version 22 was used to analyse the data. The significant change in pain and mouth opening was assessed by applying paired sample t-test. P-value &pound; 0.05 was considered as significant.<strong> </strong>The mean age of patients was 43.61 &plusmn; 12.74 years. There were 23 (33.8%) male and 45 (66.2%) female candidates. The mean duration of symptoms was 16.92 &plusmn; 4.83 days. The mean pain score of all the candidates was 5.67 &plusmn; 2.31 at baseline which was reduced significantly to 0.14 &plusmn; 0.11 after 3 months of the procedure (p &lt; 0.0001). The mean maximal mouth opening of all the candidates was 27.86 &plusmn; 4.15 mm at baseline which was improved significantly to 42.17 &plusmn; 3.19 mm after 3 months of the procedure (p &lt; 0.0001). It has been concluded that application of platelet rich plasma can be significantly effective in reducing pain while improving mouth opening and resolving the symptoms of TMJ dysfunction.</p>