Abstract : The study was conducted to evaluate the prevalence of growth hormone deficiency in children with isolated short stature. An observational study comprised (110) short children aged (3-15) year with current height > 2SD below the mean of comparable age and gender, who do not have any symptoms related to diseases that affect growth or specific dysmorphic features of genetic syndromes. Evaluation of children included detailed personal and family history, physical examination, growth analysis, radiological (bone age), laboratory tests, and growth hormone (GH) stimulation test. GH concentration of less than 10 ng/ml has been used to support the diagnosis. A low level of GH was found in (59) children, accounted for (53.6%). Males outnumbered females (39, 20) respectively with male to female ratio 1.95:1. The study showed late referral of children, 34% had first referral after the age of 10. Bone age was shown delayed for chronologic age in 66%. Celiac disease and hypothyroidism did not account for a considerable percentage of short stature in the current study (1.8, 0.9) respectively. Growth hormone deficiency is not uncommon in children with short stature. There is delay of referral of short-statured children for the diagnosis and treatment, awareness of growth monitoring helps improves referrals. Early initiation of therapy with GH may aid in achieving final adult height and lower the psychologic impacts. Further researches are recommended to detect IGF-1 and to study the consequences of GHD and the safety of the therapy