Abstract : Propolis is a promising natural product that have been widely studied and investigated for health and medical benefits. It appears as a sticky, greenish-brown compound found on the trees and evergreens formed by the sap, bee discharges and bees wax. Propolis is used to line the beehives. The high content of flavonoids in propolis has contributed tremendously to the traditional use of propolis for many illnesses. The variation in chemical and biological composition is due to the type of propolis and locations of the source. Various methods have been used for propolis optimization based on its chemical and biological properties. The side effects of chemical drugs and toxicity is one of the reasons a natural product like propolis is gaining popularity to treat various medical conditions. Propolis has been investigated for its anti-bacterial, anti-fungal, anti-tumor, antidiabetic, and as a treatment for allergy, bronchial asthma and gastric disorders. The promising results from these research areas have paved the way to characterize propolis and elucidate the pathways to explain its mechanism of action. This review will highlight propolis production, its chemical and biological composition and its main biological functions; with a special focus on propolis as a wound healing agent