Abstract : Oxidative stress is a status that can prevent people from suffering from diseases including cancer. The 8-hydroxydeoxyguanosine (8-OHdG) is a marker that reflects oxidative DNA damage in the body.  In this study, <em>Traganum nudatum</em> plants were investigated for their potential to protect cellular DNA from oxidative damage by evaluating 8-OHdG assays in cultured human lymphocytes by incubated in ethanol extract of <em>Traganum nudatum</em> (0.05, 0.1, 0.5, 1 and 2.5 mg/mL).  White Blood samples were taken from healthy volunteers and lymphocytes were isolated. The lymphocytes were then incubated in aqueous and ethanol extract of rosemary (0, 10, 100 and 1000 µg/ml). The obtained extracts at 10,100 and 1000µg/mL from aerial parts ethanolic extracts of <em>Traganum nudatum</em> significantly decreased the levels of 8-OHdG (P < 0.01). suggesting their usefulness as protective agents against oxidative DNA damage and offering pharmacological credibility to the ethnomedical traditional usage of the plant in medicine, modifying its beneficial application against free-radical-induced diseases