Abstract : Tuberculosis (TB) is a disease that makes patients suffer not only because of symptoms of the disease, but also the resultant of general deterioration in their quality of life (QoL). Quality of life among TB patients has developed as the best tool to evaluate tuberculosis therapy outcomes. The aim of this study was to evaluate QoL and related factors among TB patients. A descriptive study with cross-sectional design was performed in November to December 2019. About 156 tuberculosis patients from three lung clinics in Yogyakarta city were recruited to participate in this study. A validated questionnaire consisted of characteristics of demographic and clinical data, and Short Form-36 (SF-36) Quality of Life instrument was used. Descriptive and inferential statistics were chosen. A Mann whitney test was used to test the difference score of QoL among TB patients. The results of the current study indicated that the mean QoL scores among tuberculosis patients in referal hospital in Yogyakarta lower than the normal range (60.45 ± 12.23). The results highlighted that lowest scores for QoL were observed in the domain of vitality (56.35 ± 9.22) followed by domain of social functioning (57.34 ± 8.45). Overall, type of TB, stage of treatment, comorbid, and smoking status were significantly associated with QoL of TB patients. Our study reveals that tuberculosis and its comorbidities have physcial impact as well as social functioning of quality of life. The findings of this study can be the basis for improving TB programs in Yogyakarta in particular and Indonesia in general