Abstract : The aim of the study is to estimate the degree of sexual differentiation using direct measurements of orbit diameters on dry adult skulls, being representative for the contemporary south Indian population. 50 dry skulls of known sex (male = 25, female =25) from the department of Anatomy, Meenakshni Medical college Hospitals and Research Insitute where used for the study. Only the skulls with no apparent deformity were measured. Juvenile skulls were also excluded from the study. The following Paramaters were measured in right and left orbit of the skulls. The parameters like orbital height and width, Length of Medial wall, lateral wall, superior wall and inferior wall, orbital perimeter, orbital index, biorbital distance, interorbital distance and orbital opening area were done. Kolmogorov Smirnov test has been used to test the normality of the data. Mann Whitney U test was used to find the statistically significant difference in orbital parameters between males and females Discriminant analysis was performed to predict whether the skull belongs to male or female. P values of 0.017, <0.001, 0.009, 0.009 and <0.001 indicate that there is significant difference between males and females in Length Of Superior Wall Right, Length Of Superior Wall Left, Length Of Medial Wall Left, Length Of Lateral Wall Right and Length Of Lateral Wall Left respectively. P value of 0.029, <0.001 and 0.034 indicate that there is significant difference between males and females in Orbital Rim Perimeter (CM) Right, Orbital Rim Perimeter (CM) Left, Orbit opening area Right respectively. The discriminate function revealed a significant association between groups and all predictors, accounting for 64.9% of between group variability, although closer analysis of the structure matrix revealed two significant predictors, namely Length Of Superior Wall Left (- 0.445) and Orbital Rim Perimeter (CM) Left (–.357) with remaining parameters as poor predictors. The cross validated classification showed that overall 78.0% were correctly classified. Comparing the results from the present study with other similar studies we found that the highest accuracy for sex determination is length of superior wall left side and orbital rim perimeter on the left side.