Abstract : Mangosteen (Garcinia mangostana L.) has antioxidant, antibacterial, antitumor, anticancer, and antiproliferative functions. Previously, an acute toxicity test of the ethanolic extract of mangosteen rind had been carried out, so further testing was needed, namely the subchronic toxicity test, to determine the toxic effects that were not detected in previous studies. This study aims to assess the degree of toxicity of changes in liver function considered from the levels of SGPT and SGOT. This research is an experimental study using the Posttest Only Control Group Design method using 40 individuals. Rats were divided into the control group and treatment group. The treatment group was divided into three groups: a dose group of 250 mg/kg BW, 500 mg/kg BW, and 1000 mg/kg BW. The control group was only given drinking water and feed. Dosing is provided for 28 days. Observations were made for 28 days, and on the 29th day, blood was taken to check the SGPT and SGOT. The results showed an increase in SGPT levels in male rats at a dose of 250 mg/kg BW and SGOT levels in female rats at 250 mg/kg BW but did not cause subchronic toxicity effects on the liver damage. There was no significant difference between male and female SGPT levels using the Kruskal Wallis test (p = 0.240 and p = 0.152). The SGOT level of male rats using the Kruskal Wallis test was found to be p = 0.061, and the SGOT level of female rats using the One Way ANOVA test was obtained p = 0.101.