Abstract : Vascular endothelial growth factor (VEGF) induces proliferation of endothelial cells, stimulates angiogenesis, and increases vascular permeability, but information about its role in bone remodeling therapy is limited. The aim of this study is to determine Evaluation of the expression of macrophage colony- stimulating factor (M-CSF) to assessment for dental tissue response includes (periodontal ligament, cementum, alveolar bone) to application of LLLT and VEGF in orthodontic treatment in experimental rabbits. Immunohistochemical study for osteoblastogenesis and osteoclastogenesis include the followings Evaluation of the expression of tissue nonspecific alkaline phosphatase (ALPs) by osteoblast. Evaluation of the expression of collagen type 1 Evaluation of the expression of macrophage colony-stimulating factor (M-CSF) to assessment for dental tissue response includes (periodontal ligament, cementum, alveolar bone) to application of LLLT and VEGF in orthodontic treatment in experimental rabbits