Abstract : Periodontal disease that has been prolonged without early treatment will result in more severe and comprehensive periodontal tissue damage. Every disease in the periodontal tissues needs initial therapy that consists of scaling and root planning to prevent more severe periodontal inflammation. The study was aimed to determine the effect of initial therapy on TGF-β1 gene expression and healing response in GCF with chronic gingivitis patients. The gingival crevicular fluid (GCF) was taken using paper points before initial therapy and after initial therapy. There were 30 gingival crevicular fluid (GCF) samples and examination was performed with real-time Polymerase Chain Reaction (RT-PCR). The results showed that p < 0.05 which there was an effect of initial therapy such as scaling and root planning on the reduction of mRNA expression of TGF-β1 gene in patients with chronic gingivitis. In conclusion, there was a decrease in mRNA expression  of TGF-β1 gene in GCF with chronic gingivitis after initial therapy such as scaling and root planning for severe gingivitis which gave a healing effect in the form of changes in the gingival clinical signs