Abstract : It was suggested that the risk of prostate cancer is associated with vitamin D deficiency. In this case, one has investigated the hypothesis that the 25 (OH) D statuses affect prostate specific antigen levels. In this regard we studied the association of vitamin D blood status with serum prostate specific antigen (PSA) concentrations. A total of 491 healthy men were diagnosed through the project. We have enlisted 491 Moroccan men of good health. The vitamin D status and serum PSA have been evaluated. The deficiency of Vitamin D (less than 30 ng /ml) was found in 81.7% of the study population. The mean vitamin D level in this population was 22.5 ±9.56 ng /ml. Serum vitamin D and PSA levels, respectively, were significantly negatively associated with age (p < 0.001, 0.005), while no correlation was found linking PSA and vitamin D levels. Our results show that even in regions like ours where there is a lot of sunlight all year round, vitamin D deficiency is widespread. Nevertheless, variations in vitamin D have shown no impact on healthy men's serum PSA levels.