Abstract : Smoking epidemic remains to be one of the biggest public health issues the world has ever faced. Cigarette smoke can stimulate peroxidation of lipid and oxidation of proteins and become a risk factor for atherosclerosis and cardiovascular disease. Aloe vera has been used for medicinal purposes for centuries and reported to have potent antioxidant capacity. Considering its good potential as alternative medicine in the future, this study aimed to evaluate the effect of Aloe vera on serum lipid profile in the rat after being exposed to cigarette smoke. The experimental study was conducted on male rats which were divided into 3 groups. The first group was a control, the second group was exposed to the smoke of 8 cigarettes/ day for 30 minutes. The other group was given 1 mL/ day of Aloe vera gel an hour before the exposure of the smoke for 42 days. The blood sera were measured for lipid profile, consisting of total cholesterol, low density lipoprotein (LDL), and high density lipoprotein (HDL). The results were statistically analyzed using Kruskal-Wallis and post hoc test Mann-Whitney. There was a significant increase in serum LDL and a decrease in HDL between control and cigarette smoke group (p<0.05). A comparison of results between cigarette smoke and cigarette smoke plus Aloe vera groups showed a decreasing concentration of serum LDL (p<0.05). Aloe vera L. may prevent dyslipidemia in rats after being chronically exposed to cigarette smoke