: Juglans regia L. is considered as one of medical plants hasing biochemical activity to treat various diseases resulting from infection by some pathogenic micro-organisms. The current research was achieved to estimate and evaluate the medicinal activity of three phenolic compounds mixture containing 4- hydroxybenzaldeyhde, 5-(3-Hydroxypropyl)- 2,3-dimethoxy phenol and vanillic acid which were isolated and identified from Juglans regia L. cortex against four pathogenic fungi causing intestinal inflammatory in children. The phenolic mixture recorded values of inhibition zone diameters equal to 21, 30, 37, 51,52 and 52 mm at concentrations of 5, 10, 25, 50, 75, and 100 mg/ml respectively against growth of the pathogenic fungus represented by Candida albicans while the same concentrations gave inhibition values were 32, 39, 44, 46, 50 and 51 mm respectively towards Candida globate. In regard to Candida krusei, the phenolic mixture concentrations showed diameters of inhibition equal to 35, 41, 46, 53, 53 and 53 mm respectively while the values of same concentrations recorded inhibition values were represented by 25, 30, 35, 38, 52 and 52 mm against Candida tropicalis growth. The active phenolic metabolites compounds recorded a higher medicinal activity than some antibiotics used against these pathogens.