: Fungal infection is not life threatening to patients due to blood malignancies and as a result of intensive chemotherapy, solid tumor and the use of immunosuppressive drugs for organ transplant patients. The samples were mostly blood, sputum, urine, bronchi-alveolar lavage, CSF and wound swabs. Each sample was examined by conventional methods as wet mount and stained smears to culture on various media like Dextrose agar, brain heart infusion agar and blood agar, diagnosed by APE 20 Candida to be cultured on SDA using 20μ, 50μ [microns] concentration disks of drug amphotracin B and fluconazole 20μ for all patients, and was tested after 18 hours of incubation, rings above 20 mm considered positive while less 5 mm were all negative according to NICM standards. The commonest yeasts recovered were Cryptococcus neoformans Candida albicans,and Geotrichum candidum ,most of those isolates yeasts showed resistance to both concentration of amphotracine B disks mostly in cryptococcus neoformans, the fungal infection in patients being used in a massive way to protect from invasive fungal infections the incidence of resistant strains of various of yeasts showed a higher rate of drug resistance in vivo and in vitro as well endangering the drug use in daily practice as a safe drug in daily medical practice. Many types of yeast are resisting the broad spectrum anti-fungal drug as amphotracine-B, in vitro reflecting an alarming sign on the use of this drug in medical practice as preemptive or empirical strategies of IFI management.