: Pressure ulcers have been identified as a major burden of hospitalization worldwide, it causes much inconvenience, soreness, illness for a while, set in hospital for long time, and raise costs of nursing. Nurses and other health care providers have the biggest duty toward this problem; it has concluded and has become a fact, that the amount of awareness and positive attitude of nurses are main factors that could affect Pressure ulcers prevention. A cross-sectional descriptive study design was used to assess the effect of nurse's awareness of Pressure ulcers. The subject of this study comprised of the chosen number of staff nurses who met the following criteria: Both gender, one year of experience or more, working at high risk unit for PU. The researcher was elected (248) nurses who was chosen in patient units at King Khalid University Hospital, Riyadh, Saudi Arabia. The findings showed that nurses had a high level of knowledge, neutral level of attitudes, and a high level of practice. There was a significant relationship between knowledge and attitude, nurses’ attitude and practice. Additionally, there was a non-significant negative relationship between knowledge and practice regarding pressure ulcer Prevention. The result emphasized that there is effect of awareness level on the practice regard the pressure ulcer prevention.The researcher recommended the nurses need further training program about the pressure ulcer prevention strategies in order to develop their practice. Furthermore, the nurses must promote their positive attitudes and advance their practices.