: Repeated pregnancy failure is a serious problem in which numerous issues show a vital part. Accumulating body of evidence suggests a role for the anticardiolipin antibodies, antibrucella antibodies and hepatitis B surface antigen in the etiology of abortion. The objective of the present study was to estimation serum levels of anticardiolipin antibodies, antibrucella antibodies and hepatitis B surface antigen among the recurrent aborting women, unsolved pregnancy damage and intrauterine fatal demises (IUFD). One hundred &twenty (120) women were enrolled in this prospective case-controlled study conducted from September 2017-September 2019, at Maternity Hospital, Erbil City, Iraq. They were (60) patients with a history of three & more attacks of previous miscarriage with a history of hepatitis or brucellosis involved and investigations for the infections (CMV, rubella and toxoplasmosis) were negative and (60) healthy pregnant. All were screened for ACL-IgG, antibrucella antibodies and HBsAg in serum. Results revealed that (19/60, 31.7%) of the patients & (7/60, 11.67%) healthy control were positive for ACL-IgG with a mean concentration of 38±10.3 IU/ml in the patients group, and 11.6±4.4 IU/ml in the healthy control with highly significant at (p≤0.01). The distribution for (ACL with antibrucella & HbsAg) in the patients’ group were as such; 3/19 (15.79%), 6/19 (31.58%) respectively among the seropositive ACL patients with statically significant at (p < .05). This study concluded that Anticardiolipin constitute an important cause of recurrent mid-trimester abortion in Iraqi, women. Also, there is an increasing evidence of the human pathogenicity of Brucella organisms and hepatitis B virus, especially as agents of adverse pregnancy outcome.