: Antibiotic resistance of an organism associated dental infections in patients with Orofacial cleft is more common over the past two decades due to irrational usage of antibiotics. It seems to be a major challenge to dental professionals for treating patients. Hence, short term antibiogram study was performed by using methanolic extract of Garcinia imberti bourd against multidrug-resistant (MDR), periodontal pathogens as well as root canal isolates such as Staphylococcus sps, Enterococcus sps and some gram negative isolates porphyromonas sps in dental sample. Samples, from which yielded bacterial strains were subjected into antibiotic susceptibility profile to detect the Multi Drug Resistance, Xtreme Drug Resistance, and Pan Drug Resistance bacteria. Out of 50 samples tested,227 isolates obtained and from this 100 (35.68) MDR 50 (17.18)XDR and PDR 3(1.3% ). The most common MDR root canal isolate was Enterococcus faecalis (66%), followed by rare Staphylococcus sciuri species 46% (13.33%) resulted from periodontics infected cleft palate patient. PDR resistance only observed in gram positive isolates of cleft palate cases. Antibacterial activity of methanolic fraction extract of novel medicinal plant Garcinia imberti Bourd had shown to exhibit potent activity against the drug resistant isolates Enterococcus faecalis (P<0.001) and Staphylococcus sciuri (P<0.001). Enterococcus faecalis showing Resistant to vancomycin (72.56%) but moderate resistance showed against gentamicin (35.6%) followed by Staphylococcus sciuri exhibited the resistant (64.43%) and (25.16%) respectivelyA significantly high resistance was noted to the beta-lactam group of antimicrobials, fluoroquinolones and cotrimoxazole, both by the gram-negative bacilli (GNB) as well as gram-positive cocci (GPC. Methanolic extract fraction of Garcinia imberti bourd could be used for the discovery of phytochemicals with drastic efficacy to battle against multidrug resistant oral isolates..