: Healthy lifestyles have been reported to have a direct impact on bone health. As various lifestyle factors can affect bone health, hence the present study collected the lifestyle history using a detailed questionnaire and estimated the bone specific alkaline phosphatase (ALP) and calcium in the serum samples of young females from Gulf Medical University (GMU). Forty females aged between 18-25 years meeting the inclusion and exclusion criteria were selected for the study. Their lifestyle practices and physical activity characteristics were assessed through a validated questionnaire, followed by serum calcium and ALP estimation. It was reported that 82.5% participants reported having appropriate sun exposure, 30% were physically active, 27.5% took daily caffeine, 12.5% reported smoking, 80% stated adequate dairy product intake and 55% described daily intake of vegetables. The average BMI was 22.3, 80% had no history of bone fractures and there was no family history of bone disorders in 92.5% participants. 52.5% of the participants got their Vit-D checked recently, and out of them 68.4% reported to have low vit-D levels. The average serum calcium and ALP was found to be 9.7 mg/dl and 11.28 μg/l, respectively. However, when we compared the variables like physical activity, sun exposure timing, soft drink consumption and ethnicity with serum calcium and bone specific ALP, no significant difference was observed. However, limited physical activity and moderate BMI were also reported, hence improvement in lifestyle habits is warranted to promote healthy bones in later life..