: Hyperlipidaemia is a risk factor of cardiovascular disease which has become the main cause of mortality in the world and Indonesia. Sweet basil is a medicinal plant which contains beta-carotene, tannin, polyphenol and flavonoid, and it is considered to give effect on the lipid profile (Cholesterol total, Triglyceride, LDL and HDL). This study was carried out to prove the effect of sweet basil extract on the lipid profile of Hyperlipidaemia mice (mus musculus). This study was a True Experimental Design with the Randomized Posttest-Only Control Group design. The subject of study was 20 Balb/c male mice which were assigned to four groups: G1 (standard diet), G2 (standard diet and lard oil), G3 (standard diet, lard oil and sweet basil extract with the dosage of 0.53 g) and G4 (standard diet, lard oil and sweet basil extract with the dosage of 1.06 g) for 15 days. Lipid profile was examined using CHOD-PAP method. Cholesterol total, triglyceride, LDL were declined and HDL was increased in G3 and G4. Cholesterol total of G3 was declined 7.96, triglyceride 8.21, LDL 8.55 and an increase of HDL 15.13. Cholesterol total of G4 group was decreased 18.36, triglyceride 13.94, LDL 18.48 and an increase of HDL 23.98. The intervention of sweet basil extract for 15 days affected to a decline of cholesterol total, triglycerides, LDL and an increase of HDL. A dosage of 1.06 gr/ 20 gr body weight was the most effective dosage to hyperlipidaemia.