: With the proliferation of internet technology, the E-learning has become an essential method and new epitome that is widely used and implemented by educational institutions across the globe. The main objective of the current survey was to study the impact of E-learning on the students' academic performance. It was a facility-based and quasi-experimental research design that carried out in Najran University- college of nursing during the period from January to August 2019. By adopting a purposive sampling technique, 80 under-graduate nursing students (40 experimental groups + 40 as controls) that aged 21-24 years old had been recruited to participate in the current survey. Final exam results and a self-administered questionnaire were used for collecting data. The findings revealed that the mean scores obtained by students in the final exam by the E-learning group (Experimental) is statistically significantly higher than those for the traditional group (controls) (t=3.45, df=37, P value= 0.002). Additionally, the results showed that the mean of the students' overall satisfaction with the traditional face-to-face lectures in the control group was 6.26, while the mean of the students' overall satisfaction with E-learning in the experimental group was 8.74. The difference between students' attitudes was significant (P = 0.015) in favor of the experimental group. The key findings of the present study show a significant difference in learning outcomes besides positive attitudes between online and traditional learners which can be a viable alternative learning method for higher education. It also contributes to the current literature in the area of online instruction and E-learning..