: The decision on single layer or double layer to opt in intestinal anastomosis is matter of concern since each method has its own advantages and disadvantages. This comparative study endeavors to compare the outcome of a single layer Vs. Double layer anastomosis in small and large bowel in terms of the duration required to perform intestinal anastomosis, various morbidity and mortality paramaters. A comparative observational study was conducted on the patients undergoing resection anastomosis of bowel in the department of surgery of a tertiary care hospital in Maharashtra. A pretested proforma was used to collect relevant information (patient data, clinical findings, lab investigations, follow-up events, complications) from all the selected patients for one year [June 2021 to June 2022]. Those patients with ages less than 18 years, co-morbid diseases like sepsis, known cardiovascular event history, deranged liver function, and renal function were excluded from the study. We used random number tables generated by computer for allotment of the cases into groups. Group 1: Single layer suture; Group 2: Double layer suture. The mean duration of anastomosis among group 1 was 20.55 minutes and among group 2 was 31.32 minutes. There was a significant difference between the two means. (p<0.05) The average length of stay was 8.13 days and 8.22 days among group 1 and group 2 respectively and this difference was statistically not significant. (p>0.05) There was no significant difference between the complications among the two groups. Single layer technique had an advantage of lesser duration when compared with double layered technique in intestinal anastomosis. Although the complications rate of obstruction, wound infection and longer stay of hospital was higher in double layered group but did not yield any significant difference in the present study..