: The World Health Organization outlined effective measures and guidelines to curb the rapid spread of the highly infectious COVID-19 virus. However, the growing cases in Jordan, and other nations around the globe raises concerns on the adherence level to such guidelines. Therefore, this research study was designed to determine the level of knowledge, practices and barriers towards the COVID-19 infection prevention strategies among University Students in Northern Jordan. Accordingly, the researcher administered a survey to 501 participants for subsequent analysis. Statistical analysis using SPSS, version 25 reported adequate knowledge scores (84.4%) among the participants. There was proper adherence to general hygiene (89.2%) hand hygiene (87.7%), and respiratory hygiene (80.8%) practices. Lack of personal protective equipment and the inconvenience of staying at home are the leading barriers to stopping the spread of COVID-19. Gender, marital status, level of education and age have a statistically significant impact (p<.05) on the participants’ knowledge towards the overall prevention of the virus spread. Nonetheless, there is still need for government, through the healthcare personnel to provide the necessary equipment and also increases awareness among the university students in Jordan to prevent the spread of COVID-19.